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Inhalation Sciences AB - Breathing New Life into Inhalation R&D

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DissolvIt

DissolvIt ® enables in vitro simulation of dissolution and absorption of respirable particles in the lung epithelium. 

 

In the patented DissolvIt ® in vitro module: dry powder particles are deposited on a glass surface by PreciseInhale®. The particles are then brought into contact with a simulated lung/airway mucus and the dissolution is studied from the ‘luminal’ side through optical microscopy and from the ‘vascular’ side by chemical analysis of a flow-past perfusion medium.

Features and Benefits

  • Evenly deposits particles over test surface

  • Small amount of substance/test surface

  • Dynamic system - no recirculation or stirring beaker

  • Solubility ranking tool

  • Experiments followed in a microscope

  • More lung-like physiology simulated during the dissolution experiment

  • Controlled particle-particle interferences during dissolution 

  • Generate pharmacokinetic profiles

  • Possibility to rank test substance against each other

  • Possibility to compare pharmacokinetic profiles of drug candidates with a reference product

  • Visualization of dissolution as disappearance of particles 

  • Knowledge about particle behaviour such as hygroscopicity

The system consists of a dissolution chamber, a precision-controlled peristaltic pump and an inverted microscope with a high resolution camera. The dissolution chamber is perfused in single-pass mode after which the blood simulant is collected in a fraction collector. To further mimic the conditions in the lung, this system is heated to 37°C.

 

Particles pre-deposited on glass cover slip are sandwiched with a mucus simulant in the dissolution chamber. Dissolved particles absorbed by the single-pass perfusate are sampled by the fraction collector at selected timepoints. The dissolution process is monitored through the inverted microscope.

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